The Single-Cell Analytics Innovation Lab (SAIL) has experience with a wide range of tissue sources, and can generate different types of single-cell data using a variety of library preparation platforms.
Single-Cell Library Preparation Platforms
SAIL provides three library preparation platforms for single-cell RNA sequencing.
10x Genomics Chromium X
The SAIL workhorse. The Chromium is a droplet-based sequencing library preparation for generating single-cell genomics dataset as scRNAseq, scATACseq, Multiome, +/-CITEseq, +/-VDJ, +/- multiplexing.
![10x Genomics Chromium](/sites/default/files/node/199915/inline_images/10xgenomicschromium.jpg)
Onyx by Droplet Genomics
ONYX droplet generator combines pumps, microscope, high-speed camera and high-voltage power source into an easy-to-use package to perform and develop droplet microfluidics experiment.
![InDrop](/sites/default/files/node/199915/inline_images/indrop.jpg)
Xenium by 10X Genomics
In Situ platform enables subcellular mapping of 100s to 1,000s of RNA targets in addition to multiplexed protein, revealing new insights into cellular structure and function
![Plate-seq](/sites/default/files/node/199915/inline_images/plate-seq.jpg)
SAIL is constantly testing new technologies. We will soon offer the following sequencing library options.
- Cellular indexing of transcriptomes and epitopes (CITE-seq) — quantifies levels of multiple surface proteins (antibody-based) in addition to transcriptome measurement
Bioinformatic Support
We provide preprocessed data in the form of count matrices and Jupyter notebooks that enable SAIL users to explore clustered cell types, identify differentially expressed genes, and all other downstream analyses.
Tissues and Cell Types
SAIL generates single-cell data from diverse sources, including cell suspensions from healthy and disease tissue samples collected as clinical resections, core-needle biopsies, and fine-needle aspirates. Below are examples of tissues that we have sequenced.
Suspension cells
Mouse CD8, T-reg niche, T-reg activation and dendritic cells.
Human PBMCs, mucosal associated invariant T cells, AML (acute myelogenous leukemia), APL (acute promyelocytic leukemia), and myeloma cells, as well as lymph node and bone marrow cells.
Tissue-derived cells
Mouse brain (healthy and Alzheimer model) and thymus; lung, pancreas and prostate tumor cells.
Human healthy lung, cerebrospinal fluid, microglia, and radial-glia-like cells; prostate, breast, colorectal, ovarian, kidney, adrenal, and pancreatic tumors, lung primary tumor and metastases, leptomeningeal metastases (cerebrospinal fluid), glioblastoma, peritoneal metastases, and pleural effusion fluid.
Other tissues
Lineage-tracing mice, PDX (patient-derived xenografts), prostate and intestinal organoids, and lymphoma, lung cancer, and other tumor cell lines