The core operates a Titan Krios G2 cryo-electron microscope equipped with a Gatan K3 direct camera. The core also has an FEI Vitrorobot cryoEM sample preparation station that automates the vitrification process to provide fast and reproducible sample preparation. The facility also provides structural biology and modeling expertise and guidance to non-crystallography Memorial Sloan Kettering laboratories that benefit from the use of available structures in the design and interpretation of experiments. There is a centralized resource for crystallization protocols and reagents, including trays and the commercial Hampton and Wizard crystallization screens.

The facility has an oversight committee, which insures its proper function within the Institution. Christopher Lima serves as the Chairman of this oversight committee.