The Molecular Epidemiology Laboratory
Led by Dr. Irene Orlow, the Molecular Epidemiology Laboratory participates in self-initiated research & supports investigations from the Population Sciences Research and Clinical Research programs that involve handling and testing of a variety of human specimens.
How we do it
Experimental Approach
- Aims aligned with type of biospecimens, and short- and long-term analytical plan
- Analytical plan determines initial processing
- Sample processing plan is reviewed and technology is selected
- Assays are designed and newly developed as needed, and validated
- Quality assurance (QA) and quality control (QC) delineated
Biospecimens & Logistics
- Collection details and labeling reviewed
- Kits and instructions provided
- Delivery details and communications instructed
- Biosafety considered at each step
Biospecimens Handling & Assay Development
- Appropriate range of laboratory techniques
- Step-by step environment considerations
- Isolation
- Quality- , quantity- , and viability- assessment
- Empirical assay design
Testing & Data Generation
- Validation of assay, selection of lab controls
- Tracking and deliverables established
- Testing of Study Specimens and laboratory controls
Quality Control & Data Handling
- Review of visual data
- Data entry
- Descriptive analysis, and comparison to empirical parameters
- Data cleaning and summary
Samples Distribution & Banking
- Establishment of sample volumes and configuration or layout
- Selection of the most appropriate environmental conditions
- Aliquoting for collaborating laboratories and consortia
- Preliminary preparation for high throughput testing
- Banking for additional and/or future investigations
- Monitoring on-site, off-site, and QC