Nanopore Sequencing:
a third generation sequencing technology that enables direct, real-time analysis of long DNA or RNA fragments. It works by monitoring changes to an electrical current as nucleic acids are passed through a protein nanopore. The resulting signal is decoded to provide the specific DNA or RNA sequence along with the identification of base modifications. Using nanopore sequencing, a single molecule of DNA or RNA can be sequenced without the need for PCR amplification or chemical labeling of the sample.

References:

1) Clarke, J., et al., Continuous base identification for single-molecule nanopore DNA sequencing. Nat Nanotechnol, 2009. 4(4): p. 265-70.

2) Soneson, C., et al., A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes. Nat Commun, 2019. 10(1): p. 3359.