Description

HT-3 is a human cervical carcinoma cell line that grows in adherent culture.  Although this cell line was initially classified as human papillomavirus (HPV) DNA negative, subsequent studies revealed that the cells harbor HPV30 DNA in their genome.  The HT-3 cells have a homozygous mutation in the TP53 gene, resulting in the expression of the transactivation-defective, dominant negative form of the protein.  These cells form tumors when injected subcutaneously into immunocompromised mice.

Source

This cell line was established in 1963 from a metastatic site (lymph node) in a 53-year-old Caucasian female.

Inventors

Jorgen Fogh, PhD, formerly at Sloan Kettering Institute, Memorial Sloan Kettering

Key References

• Fogh J et al. (1977) One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. Journal of the National Cancer Institute 59: 221-226 (PubMed ID: 327080)
• Naeger LK et al. (1999) Bovine papillomavirus E2 protein activates a complex growth-inhibitory program in p53-negative HT-3 cervical carcinoma cells that includes repression of cyclin A and cdc25A phosphatase genes and accumulation of hypophosphorylated retinoblastoma protein. Cell Growth & Differentiation 10: 413-422 (PubMed ID: 10392903)
• Xiao X et al (2012) Metformin impairs the growth of liver kinase B1-intact cervical cancer cells. Gynecologic Oncology 127: 249-255 (PubMed ID: 22735790)

Licensing Information

This cell line may be licensed nonexclusively for research or commercial purposes.

• Express License:  For internal research purposes by a for-profit entity:  1) Fill out MSK’s ATCC Express License, see here for links to the online fillable ATCC Express License, as well as a PDF version.  2) After execution by MSK, you will receive a signed copy, and can then place your order with ATCC. For detailed guidance on how to fill out this webform, see here. Please note:  In order to streamline and expedite the licensing of select MSK cell lines, the terms of this non-exclusive license are non-negotiable. This license is only available for select cell lines, including this one. Once this license has been executed by MSK, you will receive a signed copy and payment instructions; all payments up to $10,000 must be made by credit card.
• Commercial License: Contact MSK’s Tangible Materials team at [email protected].
• For non-licensing requests from academic-research institutions: Frances Weis-Garcia, PhD, Associate Laboratory Member/Head, Antibody & Bioresource Core Facility, MSK, 646-888-2354, [email protected]